ABSTRACT
Objective: The goal of this investigation was to look at the frequency and dispersal of bacteria isolated from pus/wound, as well as their susceptibility patterns. Materials and Methods?A study was conducted on 175 patients who provided pus and/or wound discharge samples in different wards (outpatient department or inpatient department). MacConkey agar and blood agar plates were immediately inoculated with samples and incubated at 37°C for 24?hours. The Gram stain and biochemical tests were used to identify all isolates after incubation. Kirby–Bauer's disc diffusion method was used to perform sensitivity tests on Mueller–Hinton agar plates. Results?This study covered 175 patients, with a bacterial isolation rate of 102 (58.28%). Males outnumbered females in the samples (M:F?=?1.8:1), with a median age of 45 years as majority were in the age group of 40 to 60 years which was 41 (40.20%). Total 90.1% samples showed monomicrobial infection, whereas 9.8% showed polymicrobial infection, and total 112 bacterial strains were isolated. Conclusion?Escherichia coli was the most prevalent isolate in present investigation, followed by Pseudomonas aeruginosa. Chloramphenicol is the only antibiotic which is effective for both gram-negative bacilli and gram-positive cocci. This report's susceptibility statistic may be worth considering for developing empiric treatment regimens for pyogenic infections.
ABSTRACT
BACKGROUND- The most common cause of urinary tract infection is Escherichia coli, though other bacteria or fungi may rarely be the cause. Recurrent UTI's that are predominantly caused by uropathogenic Escherichia coli's forms biofilm. OBJECTIVES1. To determine the biofilm formation by E.coli. 2. To evaluate the antibiotic susceptibility testing. MATERIAL AND METHODS- The present prospective study was carried out from December 2014 to May 2015 in the Department of Microbiology of SMS medical college, Jaipur. A total of 100 consecutive, nonrepetitive E. coli isolates were subjected to biofilm formation study by Christensen's tube adherence method. All the isolates were also subjected to antimicrobial susceptibility testing by Kirby-Bauer disc diffusion method in accordance with the Clinical Laboratory Standard Institute 2013) guidelines and followed inclusion and exclusion criteria. Epi-info software was used for data analysis. RESULTS- Out of the 100 E. coli isolates studied, 62 (62%) were positive for biofilm formation. Overall drug resistance was found to be very high among both biofilm as well as non-biofilm forming isolates indicating excessive drug resistance among both community and hospital organisms. CONCLUSION- The results of present study indicated a need for continued surveillance of antimicrobial resistance and greater understanding of the nature of biofilm producing uropathogens causing UTI, so as to increase positive outcomes of clinical interventions and the development of novel and more effective treatments for the problematic diseases.